Journal: Immunity

Article Title: Human Antibodies Fix Complement to Inhibit Plasmodium falciparum Invasion of Erythrocytes and Are Associated with Protection against Malaria

doi: 10.1016/j.immuni.2015.02.012

Figure Lengend Snippet: Invasion Inhibition by IgG and Complement and Complement Deposition on the Merozoite Surface (A) Invasion-inhibitory activity of purified IgG from Kenya and PNG was tested in invasion assays performed with 50% normal serum (NS; complement active) or heat-inactivated serum (HIS; complement inactivated). Data represent the mean ± range from two independent assays performed in duplicate. (B) C1q and C3 deposition on merozoites incubated with purified PNG IgG, purified malaria-naive IgG (Australian donors), or PBS together with 25% NS for 1, 5, 15, and 30 min. MSP1-19, a merozoite surface protein, was used as a loading control. (C) C3b deposition on merozoites incubated with purified PNG IgG and 25% NS or HIS via immuno-electron microscopy. Gold labeling is indicated with arrows. Scale bars represent 0.1 μm. (D) Formation of the membrane attack complex (MAC; complement components C5–C9) on merozoites incubated with purified PNG or Australian (Melbourne) IgG and 25% NS or HIS via IF microscopy. (E) MAC deposition as quantified by ELISA on merozoites incubated with NS and PNG or Australian IgG. Immunoblots and microscopy images are representative of two independent experiments. See also Figure S1 .

Article Snippet: C1q and C3 were detected with anti-C1q (Goat polyclonal, Calbiochem, Merck) and anti-C3 (HRP-conjugated goat polyclonal, MP Biomedicals), respectively.

Techniques: Inhibition, Activity Assay, Purification, Incubation, Immuno-Electron Microscopy, Labeling, Microscopy, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Cytotherapy

Article Title: Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis.

doi: 10.1016/j.jcyt.2016.06.008

Figure Lengend Snippet: Figure 1. Functional and phenotypic characterization of aMSCs. (I) Exponentially growing aMSCs (n = 4) were differentiated to adipocytes (A, B, C), chondrocytes (D, E, F) and osteocytes (G, H, I). After 14–21 days, cells were fixed and saved for immunohistochemistry (IHC). (A) Represents the bright field (BF) image of the corresponding mouse FABP-4-positive newly formed adipocytes (red, FABP-4 + ve) shown in panel B. (C) The oil red O staining of the fat droplets inside newly formed adipocytes (dark red). (D) The BF image of the correspond- ing collagen II–positive (Collagen-II +ve) newly formed chondrocytes (red) shown in (E). (F) Alcian blue staining of sulphated mucin (blue) and neutral red (NR) staining of the lysozymes (red) inside newly formed chondrocytes. (G) Represents the bright field (BF) image of the corresponding osteopontin-positive newly formed osteocytes (red, Osteopontin +ve) shown in (H). (I) Represents the alizarin red staining of calcium deposits inside newly formed osteocytes (red). Images magnification is 40 × (n = 1). (II) aMSCs were analyzed by flow cytometry (FC) after being cultured for 1–7 days in both DMEM and α-MEM.We assessed the expression of surface antigens often seen on MSCs (i.e., Sca1, CD106, CD105, CD73, CD29 and CD44), as well as those absent on MSCs but typical of hematopoietic stem cells (i.e., CD11b and CD45), with the percentages of expressing cells shown on each panel. We used FITC-labeled antibodies for detecting the percentage of expressing cells. n = 4; data are presented as the mean ± SEM. (III) Represents analysis of one experiment showing the percentages of FITC-labeled surface antigens expected on both MSCs and hematopoietic stem cells.

Article Snippet: After incubation and washing, cells were resuspended in 200 μL of the buffer including 10 μL of goat F(ab′)2 anti-rat immunoglobulin G fluorescein isothiocyanate (FITC; R&D System, cat. # F0104B) for 30 min at 4°C in the dark.

Techniques: Functional Assay, Immunohistochemistry, Staining, Cytometry, Cell Culture, Expressing, Labeling